Date	Topic	Very Tentative Schedule of Activities	Team clean-up
M 1/12	Getting Started in Biotechnology The basics	Sterile Technique and Lab Safety; Exercises 1 and 2 HW# 1 due W.	1
W 1/14		Measurement of Volume and Making Stock Solutions; Exercises 1-4 HW # 2 due W	2
M1/19		No Class
W 1/21		Making Solutions Continued; Using Micropipeters Continued (Ex. 1-3) HW #3 due M Read pp. 44-45 and 47-48 for quiz M	3
M 1/26	Unit One – Protein Techniques Study of enzyme activity	QUIZ Introduction to the project Laboratory documentation and notebook keeping – need notebook, black ballpoint pen, and black Sharpie by today. Lab 1 – Starch Plate Assay - Part II - Streak bacterial cell plates (p. 52) HW # 4 due W	4
W 1/28		Lab 1 – Starch Plate Assay – Part I – Assay samples and Part II - Assay cells and Micropipetting exercise pp. 41-43. Lab 1 write up due M Read pp 55-57 for quiz M	5
M 2/2		QUIZ Lab 2 – Quantitative Enzyme Assay – Part I - Generate standard curve	6
W 2/4		Lab 2 – Quantitative Enzyme Assay – Part II - Measure enzyme activity and Part III - Quantitate protein Lab 2 write up due W 2/11 Read pp 65-66; 181-182 for quiz M	1
M 2/9		QUIZ Lab 3 – Factors affecting enzyme function: temperature and pH Lab 3 write up M 2/16 Read pp 74-75; 77-82	2

W 2/11	Analysis of proteins	Introduction to protein analysis techniques; electrophoresis and immunoblots. Practice polyacrylamide gel electrophoresis. Read pp. 82-83	3
M 2/16		QUIZ Lab 5 – Analysis of proteins Lab 5A –SDS-PAGE; prepare samples and pour gels for next lab	4
W 2/18		Lab 5A – Load & run & stain SDS-PAGE gels. Transfer proteins to membrane Read pp. 71-73	5
M 2/23		Lab 5B – Western blotting Lab 5 write up due M 3/2 End of material for Test One – Test One will be on W 3/2 Agarose gel electrophoresis – precast 0.7% gels Discussion of nuclease activity Read pp. 95; 98-100	6
W 2/25	Unit Two – DNA Techniques DNA isolation	QUIZ Lab 7 – Isolation of Chromosomal DNA from Bacillus licheniformis – DNA isolation	1
M 3/2		Lab 7 – Isolation of Chromosomal DNA from Bacillus licheniformis – Run chromosomal DNA on 0.7% agarose gel Lab 7 write up due M 3/23 Pre-Lab 8 - PCR practice – amplification of the a-amylase gene – assemble PCR reaction mixes	2
W 3/4	Test One	Solution making & protein techniques
M7-15	Spring Break

W 225	Unit Two – DNA Techniques DNA isolation	Lab 7 Isolation of Chromosomal DNA from Bacillus licheniformis	1
M 2/26		Lab 7 – Run chromosomal DNA on agarose gel	2
M7-15	Spring Break
M 3/16	Gene detection	QUIZ Run amyE PCR products on 1% agarose gel Lab 8 PCR Amplification and Labeling of Probe DNA Assemble PCR rxn mixes to make DIG-labeled amyE probe	3
W 3/18		Pour another 1% agarose gel Run DIG-PCR products on 1% agarose gel Lab 8 write up due W 3/25	4
M 3/23		QUIZ Lab 9 Southern Hybridization Lab 9A – Assemble restriction digestions of DNA for Southern Pour 0.7% agarose gel	5
W 3/25		Lab 9A – Run restriction fragments of chromosomal DNA on agarose gel. Discuss hybridizations during run Lab 9B - Denature DNA and set up capillary transfer of DNA to membrane	6
M 3/30		Lab 9B – Affix DNA to membrane. Lab 9C – Set up Southern hybridization	1
W 4/1		Lab 9C – Detect hybridized DNA. Lab 9 write up due F 4/3 by 5pm.	2
M 4/6	Test Two	DNA techniques Part A

W 4/8	Unit Three – DNA Techniques Cloning genes	QUIZ Lab 10 Cloning the a-Amylase Gene Lab 10A - Set up HindIII cleavage of chromosomal DNA Lab 10B – Set up HindIII cleavage of plasmid DNA	3
M 4/13		Lab 10A, B – Run cleaved chromosomal DNA and plasmid DNA agarose gel. Lab 10C –Assemble ligation reactions	4
W4/15		Lab 10D - Transformation	5
M4/20		Lab 10E – Primary screening: stain plates, pick colonies, dilute, spread plates	6
W 4/22	Analyzing clones	Lab 10E – Secondary screening: stain plates, pick positive colonies. Lab 10 write up due W 4/29 QUIZ Lab 11 Verification of a-Amylase Clones Lab 11A – Assemble PCR rxn mixes Pour two 1% agarose gels	1,2
M 4/27		Lab 11A – Run PCR products on agarose gel Lab 11B – Isolate plasmid DNA from clones Lab 11C – Set up BglII cleavage reaction	3,4
W4/29		Lab 11C – Run BglII-cleaved plasmid DNA on agarose gel No write up of Lab 11, we’ll just cover it on the final J	5,6
M 5/4	Final Exam	2:00 – 3:50pm BR 129